Identification of four complementary RNA species in Akabane virus-infected cells

Identification of four complementary RNA species in Akabane virus-infected cells.

The analysis of RNA extracted from purified Akabane virus demonstrated the presence of three size classes of single-stranded RNAs with sedimentation coefficients of 31S (large, L), 26S (medium, M), and 13S (small, S). Molecular weights of these RNA species were estimated to be 2.15 X 10(6), 1.5 X 10(6), and 0.48 X 10(6) for the L, M, and S RNAs, respectively. Hybridization analysis involving vi...

Macromolecular synthesis in cells infected with Herpes simplex virus

Identification of some secondary metabolites produced by four Penicillium species

Fungi produce a wide range of secondary metabolites such as antibiotics, toxins, alkaloids, fatty acids, ketones and alcohols during active cell growth. The present study was aimed to identify secondary metabolites from some Penicillium species, using GC-MS. Many important compounds such as 3-oxoquinuclidine in Penicillium jenseii, formamidine in Penicillium pusillum, orcinol and 1,3,8-p-mentha...

Akabane virus infection.

Akabane virus is a Culicoides-borne orthobunyavirus that is teratogenic to the fetus of cattle and small ruminant species. Depending upon the stage of gestation atwhich infection occurs, and the length of gestation of the mammalian host, a range of congenital defects may be observed. The developing central nervous system is usually the most severely affected, with hydranencephaly and arthrogryp...

Identification of RNA Binding Proteins Associated with Dengue Virus RNA in Infected Cells Reveals Temporally Distinct Host Factor Requirements

BACKGROUND There are currently no vaccines or antivirals available for dengue virus infection, which can cause dengue hemorrhagic fever and death. A better understanding of the host pathogen interaction is required to develop effective therapies to treat DENV. In particular, very little is known about how cellular RNA binding proteins interact with viral RNAs. RNAs within cells are not naked; r...